Amfr Knockout cell line (BHK-21)

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Amfr Knockout cell line (BHK-21) Order

Amfr Knockout cell line (BHK-21)
Catalog#
YKO-R070
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Amfr Knockout cell line (BHK-21)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
Amfr Knockout cell line (BHK-21)
Morphology
Epithelial-like, adherent
Passage ratio
1:2-1:3
Species
Gene Name
Amfr
Gene ID
101823213
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Amfr gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the BHK-21 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Amfr gene knockout Amfr Knockout cell line (BHK-21) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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